What’s Changed with Genome Editing?

Genome Editing With Targeted Deaminases

Luhan Yang, Adrian W. Briggs*, Wei Leong Chew*, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha Venkataramanan Soundararajan, Feng Zhang, George Church 1 Department of Genetics, Harvard Medical School, Boston, MA 2 Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 3 Harvard-MIT Division of Health Science and Technology...

Genome Editing



Genome editing with engineered nucleases in plants.

Numerous examples of successful 'genome editing' now exist. Genome editing uses engineered nucleases as powerful tools to target specific DNA sequences to edit genes precisely in the genomes of both model and crop plants, as well as a variety of other organisms. The DNA-binding domains of zinc finger (ZF) proteins were the first to be used as genome editing tools, in the form of designed ZF nuc...

Novel Genome Editing With CRISPR-Cas9

There are 9 Let7 family members present in keratinocytes, all acting as translational suppressors of the same target mRNAs. This redundancy implies that Let7 is crucial to cellular functioning, and it has indeed been found to act as a tumor suppressor, down regulating functions such as proliferation, cell motility, and de-differentiation. I hypothesized that increasing loss of Let7 loci would l...

Advancing Chimeric Antigen Receptor-Engineered T-Cell Immunotherapy Using Genome Editing Technologies: Challenges and Future Prospects

Chimeric antigen receptor engineered-T (CAR-T) cells also named as living drugs, have been recently known as a breakthrough technology and were applied as an adoptive immunotherapy against different types of cancer. They also attracted widespread interest because of the success of B-cell malignancy therapy achieved by anti-CD19 CAR-T cells. Current genetic toolbox enabled the synthesis of CARs ...